Product Description

Histone H3 (Mono-Methyl K80) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64173-50, BS64173-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecμles of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globμlar domains. Such modifications include methylation, citrμllination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: CKLF-like MARVEL transmembrane domain-containing protein 3, Chemokine-like factor superfamily member 3, CMTM3, CKLFSF3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Methylated

Immunogen: Synthetic peptide, corresponding to amino acids 3-63 of Human CMTM3.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Histone H3 (Mono-Methyl K80) polyclonal antibody detects endogenous levels of histone H3 only when mono-methylated at Lys80.

Molecular Weight: ~ 17 kDa

Pathway: Histone Lysine Methylation Pathway, Examples of Crosstalk Between Post-translational Modifications, DNA Methylation,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P68431/Q71DI3/P84243/Q6NXT2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only