Product Description

Histone H3 (MonoMethyl-K18) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67393-50, BS67393-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing mμltiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development . Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing . Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modμles such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) . The discovery of histone demethylases, such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1, has shown that methylation is a reversible epigenetic marker.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Pig, Zebrafish

Host: Rabbit

Applications: WB

Alternate Names: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST2H3A, HIST2H3C, H3F2, H3FM, HIST2H3D, Histone H3.2, Histone H3/m, Histone H3/o, H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B, Histone H3.3, H3F3C, Histone H3.3C, Histone H3.5

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Methylated

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3 with a site at MonoMethyl-K18. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Histone H3 with a site at MonoMethyl-K18 protein.

Molecular Weight: ~ 17 kDa

Pathway: MAPK Erk in Growth and Differentiation Pathway(，Histone Lysine Methylation Pathway，NF-kB Signaling，Signaling Pathways Activating p38 MAP Kinase.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P68431, Q71DI3, P84243, Q6NXT2

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only