Product Description

Histone H4 (Tri Methyl Lys20) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES20540-50, ES20540-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: H4K20ME3, HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4

Clonality: Polyclonal

Immunogen: Synthetic Peptide of Histone H4 (Tri Methyl Lys20)

Isotype: IgG

Target: Histone H4

Specificity: The antibody detects endogenous Histone H4 (Tri Methyl Lys20) protein.

Dilution: WB: 1:500-1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Formlation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Expression: B-cell lymphoma, Bone marrow, Brain, Clones donated by HIP, Corpus call

Observed Band (KD): 14kD

Fields: Neutrophil extracellular trap formation, Alcoholism, Viral carcinogenesis, Systemic lupus erythematosus

Function: Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
PTM: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin
PTM: Citrullination at Arg-4 by PADI4 impairs methylation
PTM: Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing
PTM: Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is p

Subcellular Location: Nucleus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: HIST1H4A

Protein Name: Histone H4

Mouse Gene ID: 100041230

Mouse SwissProt Number: P62806

Rat Gene ID: 100360950

Rat SwissProt Number: P62804

Research Use Only