Product Description

HMG-1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4232-50, ES4232-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IF, WB, IHC, ELISA

Alternate Names: HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from the N-terminal region of human HMG-1.

Isotype: IgG

Target: HMGB1,

Specificity: HMG-1 Polyclonal Antibody detects endogenous levels of HMG-1 protein.

Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous. Expressed in platelets (PubMed:11154118).

Observed Band (KD): about 30kd

Fields: Base excision repair, Autophagy - animal, Necroptosis, Neutrophil extracellular trap formation

Function: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Similarity: Belongs to the HMGB family. Similarity: Contains 2 HMG box DNA-binding domains.

Subcellular Location: Nucleus Chromosome Cytoplasm Secreted Cell membrane, Peripheral membrane protein, Extracellular side Endosome Endoplasmic reticulum-Golgi intermediate compartment In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune a

Storage and Stability: -20°C/1 year

Gene Name: HMGB1

Protein Name: High mobility group protein B1

Human Gene ID: 3146

Human SwissProt Number: P09429

Mouse Gene ID: 100862258

Mouse SwissProt Number: P63158

Rat Gene ID: 25459

Rat SwissProt Number: P63159

Research Use Only