Product Description

hnRNP K Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2551-50, ES2551-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference, it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: HNRNPK, HNRPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human hnRNP K. AA range:250-299

Isotype: IgG

Target: hnRNP K

Specificity: hnRNP K Polyclonal Antibody detects endogenous levels of hnRNP K protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Cajal-Retzius cell, Colorectal cancer and surrounding

Observed Band (KD): 51kD

Fields: Spliceosome, Viral carcinogenesis, MicroRNAs in cancer

Function: Function: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA
mass spectrometry: PubMed: 11840567,PTM: Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine
Similarity: Contains 1 KH domain
Similarity: Contains 2 KH domains
Similarity: Contains 3 KH domains
Subcellular Location: In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear
Subunit: Interacts with RBM42 and ZIK1 (By similarity). Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRNPK, HNR

Subcellular Location: Cytoplasm Nucleus, nucleoplasm Cell projection, podosome Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036) In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702)

Storage and Stability: -20°C/1 year

Gene Name: HNRNPK

Protein Name: Heterogeneous nuclear ribonucleoprotein K

Mouse Gene ID: 15387

Mouse SwissProt Number: P61979

Rat Gene ID: 117282

Rat SwissProt Number: P61980

Research Use Only