Product Description

Horse DEFb2 (Defensin Beta 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5917-48, ELK5917-96, ELK5917-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Horse

Alternate Names: B-DF2, DEFB2, BD2, DEF-B2, HBD2, DEFB102, HBD-2, SAP1, DEFB4A, DEFB4, Skin-Antimicrobial Peptide 1

Sensitivity: 29 pg/mL

Assay Type: Sandwich

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse DEFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse DEFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse DEFb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse DEFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only