Product Description

HSP27 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65955-50, MB65955-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regμlated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellμlar resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a resμlt of the activation of the p38 MAP kinase pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic mμltimers to dimers and monomers. It has been shown that phosphorylation and increased concentration of HSP27 modμlates actin polymerization and reorganization.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: HSP27, HSP28, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regμlated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein corresponding to human HSP27.

Dilution: WB (1/1000 - 1/2000), IHC (1/100 - 1/200)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of HSP27 protein.

Molecular Weight: ~ 27 kDa

Pathway: Inhibition of Apoptosis, Signaling Pathways Activating p38 MAP Kinase,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P04792

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only