Product Description

HSP27 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0362-50, AP0362-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04792

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Background: Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HSP27 polyclonal antibody detects endogenous levels of HSP27 protein.

Bioworld Molecular Weight: ~ 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSP27 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: H9C2 whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug), Western blot (WB) analysis of HSP27 (S78) pAb at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: H9C2 whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug), Immunoprecipitation of L02 whole cell lysate using HSP27 pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 20% input. The western blot was probed using HSP27 pAb #AP0362. “↑”（supernatant); “↓”（deposition）

Alternative Name: Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human HSP27.

Conjugate: Unconjugated

Modification: Unmodified