HSP40 (P303) Polyclonal Antibody-BS1178

HSP40 (P303) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1178-50, BS1178-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25685

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC/IF: 1:50~1:200
IP: 1:10~1:100

Background: Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HSP40 (P303)pAb detects endogenous levels of HSP40 protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSP40 (P303) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: SK-OVCAR3 whole cell lysate (40ug)
Lane 4: AML-12 whole cell lysate (40ug)
Lane 5: PC12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of HSP40 (P303) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of L02 cell lysate using HSP40 (P303) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4). Lane 1 is 30% input. The western blot was probed using HSP40 (P303) pAb #BS1178. “↑”（supernatant); “↓”（deposition）

Alternative Name: DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP 40; Heat shock protein 40; Human DnaJ protein 1; hDj-1; DNAJB1; DNAJ1; HDJ1; HSPF1

Immunogen: Synthetic peptide, corresponding to amino acids 270-320 of Human HSP40.

Conjugate: Unconjugated

Modification: Unmodified