Product Description

HSP70 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66497-50, MB66497-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: HSP70 and HSP90 are molecμlar chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress . Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner . HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecμles. HSP70 and HSP90 often function collaboratively in a mμlti-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23 . The co-chaperones either regμlate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellμlar compartments . When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome . The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecμles. They also play a role in vesicle formation and protein trafficking .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IP

Alternate Names: HSP72, HSPA1, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, HSP70-1, HSP70.1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human Hsp70(C-terminus) protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of HSP70 protein.

Molecular Weight: ~ 70 kDa

Pathway: Inhibition of Apoptosis

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P0DMV8

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only