Human B2-glycoprotein 1 (β2-GP1) antibody lgG ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0936-48, ELK0936-96, ELK0936-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: APOH; BG; B2G1; B2GP1
Assay Type: Sandwich
Sensitivity: 0.06 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human β2-GP1 lgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human β2-GP1 lgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human β2-GP1 lgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human β2-GP1 lgG in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only