Product Description

Human BAFF/CD257 (B-Cell Activating Factor) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0009-24T, E-CL-H0009-48T, E-CL-H0009-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: BAFF; CD257

UNIProt ID: Q9Y275

Target Synonyms: TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4;

Research Areas: Cancer, Cell Biology, Immunology

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human BAFF/CD257 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BAFF/CD257. You can calculate the concentration of Human BAFF/CD257 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human BAFF/CD257 in samples. No significant cross-reactivity or interference between Human BAFF/CD257 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag