Human C3 (Complement Component 3) CLIA Kit - E-CL-H0572

Human C3 (Complement Component 3) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0572-24T, E-CL-H0572-48T, E-CL-H0572-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: C3

UNIProt ID: P01024

Target Synonyms: ASP; CPAMD1; AHUS5; ARMD9; C3a; C3b;

Research Areas: Cancer, Immunology, Signal transduction

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human C3 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 0.94 ng/mL

Detection Range: 1.56-100 ng/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human C3. You can calculate the concentration of Human C3 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human C3 in samples. No significant cross-reactivity or interference between Human C3 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag