Human C4a (Complement Component 4a) CLIA Kit - E-CL-H0173

Human C4a (Complement Component 4a) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0173-24T, E-CL-H0173-48T, E-CL-H0173-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: C4a

UNIProt ID: P0C0L4

Target Synonyms: C4AD; C4S; CO4; CPAMD2; RG;

Research Areas: Immunology

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human C4a concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 0.19 ng/mL

Detection Range: 0.31-20 ng/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human C4a. You can calculate the concentration of Human C4a in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human C4a in samples. No significant cross-reactivity or interference between Human C4a and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag