Product Description

Human CD177 (CD177 antigen) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9330MS-48, ELK9330MS-96, ELK9330MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD177. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD177. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD177, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD177 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CD177, NB1, PRV1, CD177 antigen, Human neutrophil alloantigen 2a, HNA-2a, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, CD177

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction

Assay Length: 3.5h

Sensitivity: 27 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

UniProt: Q8N6Q3

Research Use Only