Product Description

Human E-Cad (E-Cadherin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK12001-48, ELK12001-96, ELK12001-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule, Cadherin, Epithelial

Sensitivity: 2.73 ng/mL

Assay Type: Sandwich

Standard: 500 ng/mL

Detection Range: 7.81-500 ng/mL

Sample Type: Serum, Plasma, Urine, Tissue Homogenates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Cd And Adhesion Molecule, Tumor Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P12830

Research Use Only