Human HRH3 (Histamine Receptor H3) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9117MS-48, ELK9117MS-96, ELK9117MS-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GPCR97; HH3R; G-protein coupled receptor 97
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: CD and Adhesion molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HRH3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HRH3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HRH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HRH3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9Y5N1
Research Use Only