Human IgM Polyclonal Antibody - E-AB-92900

Human IgM Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-92900-60, E-AB-92900-120, E-AB-92900-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Human IgM

Target Synonym: IGHM; AGM1; MU; VH

Research Areas: Cancer, Immunology

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P01871

Background: Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes, joining (J) genes, and diversity (D) genes. The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human IGHM

Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:200-1:1000

Calculated MW: 49kDa/51kDa

Observed MW: 80KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only