Product Description

Human LAG3 (Lymphocyte Activation Gene 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11989-48, ELK11989-96, ELK11989-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: CD223, Protein FDC

Sensitivity: 1.2 ng/mL

Assay Type: Sandwich

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: Serum,Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Tumor Immunity, Infection Immunity, Immune Molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAG3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P18627

Research Use Only