Human lambda immunoglobulin light chain (λ-IgLC) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK11270-48, ELK11270-96, ELK11270-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: lambda immunoglobulin light chain, lambda-IgLC
Assay Type: Competitive Inhibition
Sensitivity: 0.12 µg/mL
Standard: 20 µg/mL
Detection Range: 0.32-20 µg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Immunology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human λ-IgLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human λ-IgLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human λ-IgLC in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only