Human LMNA (Lamin A/C) Microsample ELISA Kit - ELK3554MS

Human LMNA (Lamin A/C) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK3554MS-48, ELK3554MS-96, ELK3554MS-5x96

Citations, Manuals and MSDS Available upon request.

Reactivity: Human

Alternative Names: C8C; C8G; CO8G_HUMAN;   Complement component C8 gamma chain; MGC142186

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Metabolic pathway; Immune molecule; Hematology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C8g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C8g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C8g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C8g in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P07360

Research Use Only