Human NGN2 (Neurogenin 2) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9125MS-48, ELK9125MS-96, ELK9125MS-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: NGN2; Neurogenin2; Math4A; Class A basic helix-loop-helix protein 8; Atoh4
Assay Type: Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Areas: Neuroscience
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NGN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NGN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NGN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NGN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9H2A3
Research Use Only