Product Description

Human NRF1 (Nuclear respiratory factor 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9189-48, ELK9189-96, ELK9189-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: alpha pal, alpha palindromic binding protein, locus control region factor 1, NFE2 related factor 1, NRF-1, Nrf1

Reactivity: Human

Sample Type: Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Epigenetics and Nuclear Signaling, Metabolism

Assay Length: 3.5h

Sensitivity: 0.06 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: Q16656

Research Use Only