Human OPI (Opiorphin) ELISA Kit - ELK9764

Human OPI (Opiorphin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9764-48, ELK9764-96, ELK9764-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Opiorphin

Assay Type: Competitive Inhibition

Sensitivity: 0.05 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Neuroscience

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPI in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q99935

Research Use Only