Human PHB (Prohibitin) ELISA Kit - ELK2981

Human PHB (Prohibitin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2981-48, ELK2981-96, ELK2981-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Epididymis luminal protein 215 ; Epididymis secretory sperm binding protein Li 54e ; HEL 215 ; HEL S 54e ; PHB1

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Tumor immunity; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PHB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P35232

Research Use Only