Product Description

Human PI3 (Elafin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9848-48, ELK9848-96, ELK9848-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PI3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PI3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Cementoin, ELAF_HUMAN, Elafin, Elafin/Skalp, Elastase Specific Inhibitor, Elastase-specific inhibitor, ES 1, ESI, Peptidase inhibitor 3, Peptidase inhibitor 3, skin derived, PI 3, PI-3, PI3, Pre elafin, Protease inhibitor 3 skin derived, Protease inhibitor 3, skin derived (SKALP), Protease inhibitor WAP3, SKALP, Skin derived Anti leukoproteinase, Skin-derived antileukoproteinase, Trappin 2, WAP four disulfide core domain 14, WAP four disulfide core domain protein 14, WAP four-disulfide core domain protein 14, WAP3, WFDC14

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates

Assay Type: Sandwich

Research Areas: Enzyme and Kinase

Assay Length: 3.5h

Sensitivity: 2.8 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

UniProt: P19957

Research Use Only