Human PNX-20 (Phoenixin-20) ELISA Kit - ELK8899

Human PNX-20 (Phoenixin-20) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8899-48, ELK8899-96, ELK8899-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Small integral membrane protein; Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa; MITRAC7; Phoenixin-14; PNX-14; Phoenixin-20; PNX-20; SMIM20; C4orf52

Assay Type: Sandwich

Sensitivity: 7.3 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNX-20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNX-20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNX-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNX-20 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8N5G0

Research Use Only