Human PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit - ELK1631

Human PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1631-48, ELK1631-96, ELK1631-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Developmental science; Bone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P37231

Research Use Only