Human SQSTM1 (Sequestosome 1) ELISA Kit - ELK3211

Human SQSTM1 (Sequestosome 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK3211-48, ELK3211-96, ELK3211-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: OSIL; PDB3; ZIP3; p62; p62B; p60; A170; Paget Disease Of Bone 3; Oxidative Stress Induced Like; EBI3-associated protein of 60 kDa; Ubiquitin-binding protein p62

Assay Type: Sandwich

Sensitivity: 0.108 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SQSTM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SQSTM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SQSTM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SQSTM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q13501

Research Use Only