Product Description

iNOS Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65564-50, BS65564-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellμlar response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrμlline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodμlin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimμlated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimμlating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regμlated by calcium/calmodμlin (in contrast with mouse NOS2).

Category: Primary Antibody

Reactivity: Rat

Host: Rabbit

Applications: IHC-P

Alternate Names: i NOS, Nitric Oxide Synthase, Inducible, HEP NOS, Hepatocyte NOS, HEPNOS, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, INOS, Inosl, MAC NOS, Macrophage NOS, Nitric oxide synthase 2 inducible macrophage, Nitric oxide synthase 2A (inducible hepatocytes), Nitric oxide synthase inducible, NOS 2, NOS 2A, NOS, Nos II, NOS type II, Nos2, NOS2A, NOS2_HUMAN.

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH conjugated synthetic peptide derived from mouse iNOS:811-910/1153

Dilution: IHC-P=1:400-800 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations shoμld be determined by the end user.

Purification: affinity purified by Protein A

Specificity: iNOS polyclonal Antibody detects endogenous levels of iNOS protein.

Molecular Weight: ~130kDa

Pathway: Hypoxia Signaling, Immune Cell Signaling, Insμlin &Glucose Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P29477

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only