Product Description

IRAK-1 (phospho Thr209) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5914-50, ES5914-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: IRAK1, IRAK, Interleukin-1 receptor-associated kinase 1, IRAK-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr209. AA range:175-224

Isotype: IgG

Target: IRAK-1

Specificity: Phospho-IRAK-1 (T209) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T209.

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2,

Observed Band (KD): 77kD

Fields: MAPK signaling pathway, NF-kappa B signaling pathway, Toll-like receptor signaling pathway, Neurotrophin signaling pathway, Alcoholic liver disease, Pathogenic Escherichia coli infection, Salmonella infection, Pertussis, Yersinia infection, Leishmaniasis, Chagas disease, Toxoplasmosis, Tuberculosis, Hepatitis B, Measles, Herpes simplex virus 1 infection, Epstein-Barr virus infection, Human immunodeficiency virus 1 infection, Coronavirus disease - COVID-19, Lipid and atherosclerosis

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Cofactor: Magnesium
Function: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1
PTM: Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily
Similarity:

Subcellular Location: Cytoplasm Nucleus Lipid droplet Translocates to the nucleus when sumoylated RSAD2/viperin recruits it to the lipid droplet (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: IRAK1

Protein Name: Interleukin-1 receptor-associated kinase 1

Mouse SwissProt Number: Q62406

Research Use Only