Product Description

IRF3 (Phospho-S386) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94024-50, BS94024-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14653(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF

All Applications: WB:1:1,000
ICC:1:50-1:200

Background: Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Phospho-IRF3 (S386) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0. 25% Triton X100/PBS.

Bioworld Molecular Weight: 47 kDa

Specificity: IRF3 (Phospho-S386) polyclonal antibody detects endogenous levels of IRF3 protein only when phosphorylated at S386.

Alternative Name: IRF-3, IRF3, IIAE7, Interferon regulatory factor 3, IRF 3, IRF-3, IRF3, IRF3, MGC94729,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser386 of human IRF3.

Conjugate: Unconjugated

Modification: Phosphorylation