ITLN1 Polyclonal Antibody-BS8792

ITLN1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8792-50, BS8792-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WWA0

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: Intelectin-1(ITLN1) is a secreted protein and contains 1 fibrinogen C-terminal domain. Intelectin-1 is a 40 kDaCa-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell typesand appears to participate in insulin signaling and microbe recognition. The protein has no effect on basalglucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. It increases AKT phosphorylationin the absence and presence of insulin and it may play a role in the defense systemagainst microorganisms. Italso may specifically recognize carbohydrate chains of pathogens and bacterial components containinggalactofuranosy l residues, in a calcium-dependent manner.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 37kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ITLN1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded mouse intestin using ITLN1 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of rat intestine using ITLN1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse colon using ITLN1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: ITLN1; HL-1; HL1; INTL; ITLN; LFR; hIntL; omentin

Immunogen: Recombinant fusion protein of human ITLN1(NP_060095.2).

Conjugate: Unconjugated

Modification: Unmodified