Product Description

JNK1/2/3 (phospho Thr183) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1349-50, ES1349-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spl

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mi

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Thr183. AA range:151-200

Isotype: IgG

Target: JNK1/2/3,

Specificity: Phospho-JNK1/2/3 (T183) Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at T183.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Epithelium, Fetal brain, Lung, Pooled, Testis,

Observed Band (KD): 54kD

Fields: Endocrine resistance, MAPK signaling pathway, ErbB signaling pathway, Ras signaling pathway, cAMP signaling pathway, FoxO signaling pathway, Sphingolipid signaling pathway, Mitophagy - animal, Autophagy - animal, Protein processing in endoplasmic reticulum, Apoptosis, Apoptosis - multiple species, Necroptosis, Wnt signaling pathway, Osteoclast differentiation, Focal adhesion, Tight junction, Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, C-type lectin receptor signaling pathway, IL-17 signaling pathway, Th1 and Th2 cell differentiation, Th17 cell differentiation, T cell receptor signaling pathway, Fc epsilon RI signaling pathway, TNF signaling pathway, Neurotrophin signaling pathway, Retrograde endocannabinoid signaling, Dopaminergic synapse, Inflammatory mediator regulation of TRP channels, Insulin signaling pathway, GnRH signaling pathway, Progesterone-mediated oocyte maturation, Pr

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1. JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP

Subcellular Location: Cytoplasm Nucleus Cell junction, synapse In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity). Colocalizes with POU5F1 in the nucleus.

Storage and Stability: -20°C/1 year

Gene Name: MAPK8/9/10

Protein Name: Mitogen-activated protein kinase 8/9/10

Human Gene ID: 5599/5601/5602

Human SwissProt Number: P45983/P45984/P53779

Mouse Gene ID: 26419/26420

Rat Gene ID: 116554/50658/25272

Rat SwissProt Number: P49185/P49186/P49187

Research Use Only