[KD Validated] RHOT1/MIRO1 Polyclonal Antibody-BS78314

[KD Validated] RHOT1/MIRO1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78314-50, BS78314-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IXI2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 75KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Western blot analysis of Rat testis, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Western blot analysis of extracts from wild type and RHOT1/MIRO1 knockdown 293T cells, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: RHOT1; ARHT1; MIRO-1; MIRO1; mitochondrial Rho GTPase 1

Immunogen: Recombinant fusion protein of human RHOT1/MIRO1RHOT1/MIRO1.

Conjugate: Unconjugated

Modification: Unmodified