Product Description

KDM1A Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66473-50, MB66473-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Lysine-specific demethylase 1 (LSD1, also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor. Gene activation and repression is specifically regμlated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of mμltiple histone methyltransferase complexes. In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 . LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells. In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation . Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine.

Category: Primary Antibody

Reactivity: Human, Monkey

Host: Mouse

Applications: WB, IF/ICC, IP

Alternate Names: AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of KDM1A protein.

Molecular Weight: ~ 110 kDa

Pathway: Histone Lysine Methylation Pathway

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O60341

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only