KLF4 Monoclonal Antibody-MB66723

KLF4 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66723-50, MB66723-100

Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: O43474

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC, FC

All Applications: WB (1/1000 - 1/2000)

Background: KLF4 is a member of the erythroid Kruppel-like factor (EKLF) multigene family that is highly expressed in the differentiating layers of the epidermis. KLF4 plays a critical role in the differentiation of epithelial cells and is essential for normal gastric homeostasis. Depending on the target gene, KLF4 can function as both a repressor and activator of transcription. Research studies suggest this protein may function as either a tumor suppressor or an oncogene depending on tumor type, with up-regulation in human squamous cell carcinoma of the head and neck and down-regulation in colorectal carcinoma. The in vitro reprogramming of somatic cells to an embryonic-like state has been achieved by retroviral transduction of four factors: Oct-3/4, Sox2, c-Myc, and KLF4. These induced pluripotent stem cells (iPS) are of great therapeutic interest as they exhibit the key characteristics and growth properties of pluripotent stem cells.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of KLF4 protein.

Bioworld Molecular Weight: ~ 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of KLF4 expression in recombincant protein (A) whole cell lysates., Immunofluorescent analysis of KLF4 staining in Hela cells transfected with pMX constructs. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: EZF; GKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor

Immunogen: Recombinant fusion protein of human KLF4. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified