[KO Validated] βIII-Tubulin Polyclonal Antibody-BS77077

[KO Validated] βIII-Tubulin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77077-50, BS77077-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13509

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 55kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using βIII-Tubulin/β3-Tubulin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of extracts from normal and βIII-Tubulin/β3-Tubulin Rabbit pAb knockout HeLa cells, using βIII-Tubulin/β3-Tubulin Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of Mouse brain using TUBB3 antibody at dilution of 1: 100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of Rat brain using TUBB3 antibody at dilution of 1: 100. Blue: DAPI for nuclear staining.

Alternative Name: CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4; beta-4; beta Tubulin III; TUBB3

Immunogen: Recombinant fusion protein of human βIII-Tubulin/β3-TubulinβIII-Tubulin/β3-Tubulin.

Conjugate: Unconjugated

Modification: Unmodified