[KO Validated] APEX1/APE1 Polyclonal Antibody-BS6009

[KO Validated] APEX1/APE1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6009-50, BS6009-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27695

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100 | IP,1:50 - 1:200

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 39kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using APEX1/APE1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Western blot analysis of extracts from normal and APEX1/APE1 knockout 293T cells, using APEX1/APE1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of HeLa cells using APEX1/APE1 antibody. Blue: DAPI for nuclear staining., Immunofluorescence analysis of A549 cells using APEX1/APE1 antibody.

Alternative Name: APEX1; APE; APE1; APEN; APEX; APX; HAP1; REF1

Immunogen: Recombinant fusion protein of human APEX1/APE1(NP_542380.1).

Conjugate: Unconjugated

Modification: Unmodified