Product Description

[KO Validated] HIF1AN/FIH1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS7590-50, BS7590-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD. Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regμlates NOTCH1 activity, accelerating myogenic differentiation. Positively regμlates ASB4 activity, promoting vascμlar differentiation.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF/ICC, IP

Alternate Names: HIF1AN, FIH1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human HIF1AN/FIH1(NP_060372.2).

Dilution: WB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:200 IP, 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Molecular Weight: 40kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NWT6

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only