[KO Validated] MEK2 Polyclonal Antibody-BS75946

[KO Validated] MEK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75946-50, BS75946-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P36507

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:100 - 1:200 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 45KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts from normal and MEK2 knockout HeLa cells, using MEK2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Western blot analysis of extracts of various cell lines, using MEK2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of NIH/3T3 cells using MEK2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using MEK2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: CFC4; MAPKK2; MEK2; MKK2; PRKMK2; MAP2K2

Immunogen: A synthetic peptide of human MEK2(NP_109587.1).

Conjugate: Unconjugated

Modification: Unmodified