[KO Validated] p53 Polyclonal Antibody-BS80331

[KO Validated] p53 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80331-50, BS80331-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04637

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF/ICC, IP, , ChIP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200 | ChIP,1:50 - 1:200

Background: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 53KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using p53 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Western blot analysis of extracts from wild type and p53 knockout 293T cells, using p53 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] p53 Rabbit pAb at dilution of 1:300. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Alternative Name: TP53; BCC7; LFS1; P53; TRP53; tumor protein p53

Immunogen: Recombinant fusion protein of human p53.

Conjugate: Unconjugated

Modification: Unmodified