[KO Validated] SAMHD1 Polyclonal Antibody-BS70922

[KO Validated] SAMHD1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70922-50, BS70922-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y3Z3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:50 - 1:200

Background: This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 72kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SAMHD1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Western blot analysis of extracts from normal and SAMHD1 knockout HeLa cells, using SAMHD1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min., Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] SAMHD1 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SAMHD1 antibody. Western blot was performed from the immunoprecipitate using SAMHD1 antibody at a dilution of 1:1000.

Alternative Name: SAMHD1; CHBL2; DCIP; HDDC1; MOP-5; SBBI88

Immunogen: Recombinant fusion protein of human SAMHD1(NP_056289.2).

Conjugate: Unconjugated

Modification: Unmodified