[KO Validated] SQSTM1/p62 Polyclonal Antibody-BS80343

[KO Validated] SQSTM1/p62 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80343-50, BS80343-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13501

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 62KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s., Western blot analysis of extracts from wild type and SQSTM1/p62 knockout 293T cells, using SQSTM1/p62 antibody at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s., Immunohistochemistry of paraffin-embedded Human lung adenocarcinoma using SQSTM1/p62 antibody at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Human extranodal NK-T cell lymphoma using SQSTM1/p62 antibody at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: SQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B; sequestosome-1

Immunogen: Recombinant fusion protein of human SQSTM1/p62SQSTM1/p62.

Conjugate: Unconjugated

Modification: Unmodified