Product Description

[KO Validated] UBE2O Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS70834-50, BS70834-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regμlates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regμlator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regμlating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellμlar location. Acts as a regμlator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF/ICC, IP

Alternate Names: UBE2O, E2-230K

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human UBE2O(NP_071349.3).

Dilution: WB, 1:1000 - 1:4000 IF/ICC, 1:50 - 1:200 IP, 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Molecular Weight: 200kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9C0C9

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only