Product Description

Ksr-1 (phospho Ser392) Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA26729-50, RA26729-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data., function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity., PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated., similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family., similarity:Contains 1 phorbol-ester/DAG-type zinc finger., similarity:Contains 1 protein kinase domain., subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery., subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.,

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC-p, IF (paraffin section), ELISA

Alternate Names: KSR1, KSR, Kinase suppressor of Ras 1

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight: 115

Protein Molecular Weight (kDa): 115

Storage: -20°C/1 year

Human Gene ID: 8844

Human SwissProt Number: Q8IVT5

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only