Product Description

Ksr-1 (phospho Ser392) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1473-50, ES1473-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.,PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.,subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.,

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: KSR1, KSR, Kinase suppressor of Ras 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human KSR around the phosphorylation site of Ser392. AA range:358-407

Isotype: IgG

Target: Ksr-1

Specificity: Phospho-Ksr-1 (S392) Polyclonal Antibody detects endogenous levels of Ksr-1 protein only when phosphorylated at S392.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Epithelium, Platelet, Synovial membrane,

Observed Band (KD): 115kD

Fields: Ras signaling pathway, C-type lectin receptor signaling pathway, Tuberculosis

Function: Caution: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data
Function: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity
PTM: Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated
Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family
Similarity: Contains 1 phorbol-ester/DAG-type zinc finger
Similarity: Contains 1 protein kinase domain
Subcellular Location: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestr

Subcellular Location: Cytoplasm Membrane, Peripheral membrane protein Cell membrane, Peripheral membrane protein Cell projection, ruffle membrane Endoplasmic reticulum membrane In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery

Storage and Stability: -20°C/1 year

Gene Name: KSR1

Protein Name: Kinase suppressor of Ras 1

Mouse Gene ID: 16706

Mouse SwissProt Number: Q61097

Research Use Only