Product Description

Kv3.4 (R11) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3728-50, BS3728-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q03721

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC/IF: 1:50~1:200
IP: 1:10~1:100

Background: KCNC4 (Potassium voltage gated channel subfamily C member 4) belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Kv3.4 (R11) polyclonal antibody detects endogenous levels of Kv3.4 protein.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of KV3.4 (R11) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HepG2 whole cell lysate (40ug)
Lane 3: Hela whole cell lysate (40ug)
Lane 4: PMVEC whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Kv3.4 (R11) pAb in paraffin-embedded human brain tissue., Immunoprecipitation of A549 cell lysate using KV3.4 (R11) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using KV3.4 (R11) #BS3728. “↑”（supernatant); “↓(deposition）

Alternative Name: Potassium voltage-gated channel subfamily C member 4; KSHIIIC; Voltage-gated potassium channel subunit Kv3.4; KCNC4; KCNC 4

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human KV3.4.

Conjugate: Unconjugated

Modification: Unmodified