Product Description

L-Lactic Acid (LA) Colorimetric Assay Kit

Sizes: 50Assays, 100Assays

Catalogue Numbers: E-BC-K044-S-50, E-BC-K044-S-100

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Using NAD+as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.

Abbreviation: LA

Research Areas: Glycolysis and Lipid Metabolism;Energy Metabolism

Result Type: Quantitative

Detection Instrument: Spectrophotometer

Detection Type: Colorimetric method

Detection Range: 0.05-6.0 mmol/L

Tested Sample Types: Serum;Plasma;Cell culture supernatant;Tissue;Cell

Assay Length: 1 h

Sensitivity: 0.05 mmol/L

Inter cv: 1.9%

Intra cv: 1.1%

Research Use Only