L-Lactic Acid (LA) Colorimetric Assay Kit
Sizes: 50 Assays, 100 Assays
Catalogue Numbers: E-BC-K044-S-50, E-BC-K044-S-100
Citations, Manuals and MSDS Available upon request.
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Quantitative
Detection Instrument: Spectrophotometer
Tested Sample Types: Serum, plasma, cell culture supernatant, tissue, cells
Assay Time: 60 min
Detection Type: Colorimetric method
Sensitivity: 0.05 mmol/L
Detection Range: 0.05-6.0 mmol/L
Test Principle: Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.
Inter CV: 1.9
Intra CV: 1.1
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag