Product Description

Lactoferrin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66784-50, MB66784-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P02788

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/4000)

Background: Ferritin and transferrins manage necessary iron-binding fuctions for iron metabolism. Transferrins comprise a class of single-chain, two-sited, metal-binding proteins expressed throughout the fluid and cells of vertebrates. The three major types of transferrin include serotransferrin, lactotransferrin (lactoferrin) and ovotransferrin. Lactoferrin is found in milk, tears and leukocytes. It degrades an IgA1 protease secreted by Haemophilus influenzae and, consequently, allows the human IgA1 antibody to effectively abolish Haemophilus influenzae colonization. Lactoferrin also attenuates the pathogenic potential of Haemophilus influenzae by proteolytic degradation of the Hap adhesin. While lactoferrin may aid in the the transmission of human T cell leukemia virus type 1, it inhibits HIV-1 replication at the level of viral fusion and entry into cells. The inhibitory effects of lactoferrin on mixed lymphocyte reactions suggest that it may have the ability to sense the activation status of lymphocytes. The gene encoding human lactoferrin maps to chromosome 3q21.31.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Lactoferrin protein.

Bioworld Molecular Weight: ~ 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Lactoferrin expression in K562 (A) whole cell lysates., Immunofluorescent analysis of Lactoferrin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Alternative Name: GIG12; LF; Lactotransferrin; Lactoferrin; Growth-inhibiting protein 12; Talalactoferrin

Immunogen: Recombinant fusion protein of human Lactoferrin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified